Method and test pack for the demonstration and determination of

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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23230B, 422 61, 424 12, 435810, G01N 3354

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active

043438962

ABSTRACT:
A method for the demonstration and determination of an antigen or antibody in a test fluid, and to a test kit for the application of this method. The determination of the antigen or antibody is performed by utilizing two antibodies raised against the same antigen, but in different animal species. One of the antibodies is labeled. In order to separate the labeled-bound fraction from the labeled-free fraction, an insolubilized antibody directed against the unlabeled antibody is employed. For antigen determination, the unlabeled antibody is the antibody produced from the second animal species; for antibody determination, the unlabeled antibody can be either the antibody to be detected or determined or the antibody produced from the second animal species.

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