Method and test kit for detecting an aflatoxin B.sub.1 and G.sub

Details Method and test kit for detecting an aflatoxin B.sub.1 and G.sub Method and test kit for detecting an aflatoxin B.sub.1 and G.sub

1986-10-31

1989-05-30

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 68, 4351722, 43524026, 43524027, 435810, 436518, 436548, 436808, 424 858, 530387, 530808, 530809, 935 89, 935 90, 935 95, 935103, 935104, 935106, 935110, G01N 3353, G01N 33577, C12N 1500, C12N 500

Patent

active

048351008

ABSTRACT:
Novel monoclonal antibodies to an aflatoxin B.sub.1 and G.sub.1 in a test kit and used in a method of testing are described. The method for producing the monoclonal antibodies uses repeated administration of aflatoxin B.sub.1 or the related analog compound as a 1-position polypeptide to a murine and production of a hybridoma to generate the novel monoclonal antibodies. The novel antibodies have limited cross-reactivity to aflatoxins B.sub.2, G.sub.2 and M.sub.1. Aflatoxin B.sub.1 or aflatoxin G.sub.1 are detected in foods and the like using the test kit and method.

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