Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1980-04-18
1982-07-27
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C12N 906, C12Q 126, C12R 101
Patent
active
043418686
ABSTRACT:
The substrate for xanthine oxidase such as xanthine, hypoxanthine, guanine, 2-hydroxypurine, etc. are determined by oxidizing the substrate by the action of xanthine oxidase derived from a microorganism and determining the formed hydrogen peroxide by the system for the determination of hydrogen peroxide comprising peroxidase and coloring reagent.
The determination is carried out in one step by using the test composition for the determination of the substrate for xanthine oxidase which comprises xanthine oxidase and a system for the determination of hydrogen peroxide.
The determination method can be applied to the determination of the compound which participates in the system wherein the substrate is stoichiometrically formed by the reaction or decomposition. When the system is an enzymatic reaction, the compound is determined in one step.
Xanthine oxidase used in the present invention is different from known xanthine oxidase in molecular weight and is prepared by culturing a microorganism belonging to the genus Enterobacter.
REFERENCES:
patent: 3099605 (1963-07-01), Free
patent: 4105800 (1978-08-01), Jahns et al.
patent: 4246341 (1981-01-01), Zikakis
Hashimoto, "A New Spectrophotometric Assay Method of Xanthine Oxidase in Crude Tissue Homogenate", Anal. Biochem., vol. 62, No. 2, (1974), pp. 426-435.
Fried et al., "Xanthine Oxidase", (Xanthine Dehydrogenase), Methods of Enzymatic Analysis, Bergmeyer ed., Academic Press, N.Y., (1974), pp. 644-649.
Fried et al., "Hypoxanthine and Xanthine Colorimetric Assay", Methods of Enzymatic Analysis, Bergmeyer, ed., Academic Press, N.Y., (1974), pp. 1941-1945.
Nakanishi et al., "Fermentative Production of Uricase", Chem. Abstracts, vol. 90, No. 23, p. 500, (1979) Absts. No. 184922g.
Jorgensen, "Hypoxanthime and Xanthine UV-Assay", Methods of Enzymatic Analysis, Bergmeyer, ed. Academic Press., N.Y. (1974), pp. 1941-1945.
Bergmeyer H. U.: "Methoden der Enzymatischen Analyse", vol. 1, 1974, pp. 682-688, vol. 2, 1974, pp. 1957-1963, 1976-1982, 1988-1998.
Machida Yozo
Nakanishi Toru
Kyowa Hakko Kogyo Co. Ltd.
Wiseman Thomas G.
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