Method and test composition for determination of lipid peroxide

Details Method and test composition for determination of lipid peroxide Method and test composition for determination of lipid peroxide

1987-02-04

1989-07-25

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Lipids, triglycerides, cholesterol, or lipoproteins

422 56, 435 28, 436 66, 436124, 436125, 436135, 436164, 436904, G01N 2178, G01N 3392

Patent

active

048513539

ABSTRACT:
A method for the determination of peroxide in a sample is disclosed which comprises reacting the peroxide with a chromogen represented by the general formula (I) or (II) ##STR1## wherein R.sub.1 and R.sub.3 represent amino, mono- or di- substituted amino, hydroxyl or hydroxyalkyl, R.sub.4 and R.sub.5 represent hydrogen, alkyl, alkylene, acyl, halogen, sulphone, nitro, carboxyl, hydroxyl or hydroxyalkyl, R.sub.2 represents hydrogen, ##STR2## wherein R.sub.6 represents hydrogen, alkyl, aralkyl, alkylene, aryl or mono- or di- substituted aryl, and --Z-- may change to --Z.dbd. by resonance and represents --S--, --O--, --N.dbd., ##STR3## wherein R.sub.7, R.sub.8, R.sub.9 and R.sub.10 have the same significance as R.sub.6 in the presence of heme compound, iodide or bromide and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for the determination of peroxide which comprises a chromogen as defined above and a compound selected from a heme compound, iodide and bromide.

REFERENCES:
patent: 3104209 (1963-09-01), Scott
patent: 3654179 (1972-04-01), Bauer
patent: 3713986 (1973-01-01), Bergmeyer et al.
patent: 3988208 (1976-10-01), Werner et al.
patent: 4141688 (1979-02-01), Morris et al.
patent: 4295853 (1981-10-01), Kasahara et al.
patent: 4384042 (1983-05-01), Miike et al.

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