Method and test composition for determination of enzyme activity

Details Method and test composition for determination of enzyme activity Method and test composition for determination of enzyme activity

1988-11-14

1993-03-23

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 25, C12Q 156, C12Q 126

Patent

active

051963124

ABSTRACT:
Disclosed is a method for determination of an activity of .gamma.-glutamyl transpeptidase, leucine aminopeptidase, alanine aminopeptidase, cystine aminopeptidase, X factor as a coagulation factor, thrombin, plasmin of plasminogen series, kallikrein, chymotrypsin, alkali phosphatase, N-acetyl glucosaminase and amylase, by allowing a particular substrate to act on the enzyme to thereby form an enhancer; oxidizing a chromogen by an oxidase in the presence of the enhancer and oxygen to form a pigment; and determining the pigment. Also disclosed is a test composition for carrying out the determination.

REFERENCES:
patent: 4675290 (1987-06-01), Matsumoto et al.
patent: 4681841 (1987-07-01), Matsumoto et al.
patent: 4828983 (1989-05-01), McClune

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