Method and system for the analysis of co-localizations

Details Method and system for the analysis of co-localizations Method and system for the analysis of co-localizations

2007-10-16

2007-10-16

Hannaher, Constantine (Department: 2884)

Radiant energy

Luminophor irradiation

Reexamination Certificate

active

10984627

ABSTRACT:
A method and a system for the analysis of co-localizations of dyes present in a specimen. The fluorescence spectra of the dyes present in the specimen are determined. A tolerance region around each of the fluorescence spectra is selected. The spectra of the specimen, in which at least two dyes are present, are then acquired pixel by pixel. Those spectra that lie within the tolerance region around the fluorescence spectra are then calculated. A lambda vector is calculated for each pixel and assigned to a spectrum. Images can be displayed in accordance with the assignment to the spectra.

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