Method and system for synthesizing oligonucleotides using nucleo

Details Method and system for synthesizing oligonucleotides using nucleo Method and system for synthesizing oligonucleotides using nucleo

1997-07-28

2000-08-15

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352831, C08F 200

Patent

active

061034673

ABSTRACT:
A plurality of oligonucleotides are synthesized at a plurality of locations on a substrate using a plurality of dispensing bars. Each of the plurality of dispensing bars has a respective plurality of dispensing heads arranged along a respective axis. Each of the plurality of dispensing bars is operative to selectively deposit a volume of a respective one of a plurality of nucleotide bases in any of a row of locations. A positioning mechanism positions the substrate with respect to the plurality of dispensing bars. A controller controls the plurality of dispensing bars and the positioning mechanism so that, at each of the plurality of locations, a respective sequence of nucleotide bases is deposited to form a respective oligonucleotide.

REFERENCES:
patent: 5429807 (1995-07-01), Matson et al.

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