Method and system for sorting and separating particles

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

Reexamination Certificate

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Details

C436S524000, C436S527000, C436S544000, C436S824000, C435S007100, C435S007930, C435S007910, C568S362000, C530S812000, C106S160100

Reexamination Certificate

active

07074622

ABSTRACT:
The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle and at least one peroxidase enzyme, contacting a gelatin surface with the suspending solution, adding developer to the suspending solution in contact with the gelatin surface and in the presence of phenol to attach the target particle to the gelatin surface, and washing the gelatin surface to remove unattached particles. The method may also include detecting the presence of the target particle on the gelatin surface as well as detaching and recovering the attached particles after removal of the non-target particles.

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Co-pending U.S. Appl. No. 10/989,072, Qiao et al., Method and system for multiple biological analytes detectioin (D-87468).
Co-pending U.S. Appl. No. 10/989,062, Qiao et al., Method and system for nucleic acid detection (D-87466).
M. Han, X. Gao, J.Z. Su and S. Nie,Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules,Nature Biotech. vol. 19, pp. 631-635 (2001).

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