Method and system for determining bioactive substances

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

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435 13, 435 15, 435 16, 435 4, 435 24, 435 25, 435 26, 435288, 204403, C12Q 154, C12Q 126, C12Q 100, C12M 140

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active

054118662

ABSTRACT:
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic acid. Prior to enzymatic degradation and amperometric detection of the products or elements resulting from the degradation, the interfering substances are retained by an anion exchanger disposed upstream of the enzymatic degradation site. The conditions of the method are controlled to utilize the difference of isoelectric points of the measured biosubstance and of the interfering compounds respectively.

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Hendry et al; Jour of Biotechnology, 15 (1990) 229-238.
Cattaneo et al; Biosensors & Bioelectronics 7 (1992) 329-324.
Clark, Leland C.; The Enzyme Electrode, Biosensors: Fundamentals & Applications, Turner, Karube & Wilson, eds., Oxford Science Publications, 1987, Oxford, pp. 1-12.
Chem. Abstract 118(25): 251031k (Male et al) 1992.

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