Method and system for analysis of peptides and proteins

Details Method and system for analysis of peptides and proteins Method and system for analysis of peptides and proteins

1992-06-05

1993-10-19

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436177, 436161, 422 681, 422 70, 530334, 530344, 530402, 530412, G01N 3368

Patent

active

052544761

ABSTRACT:
A method for analysis of peptides and proteins. A peptide or protein is exposed to a coupling reagent and a buffer. The coupling reagent derivatizes the terminal amino acid residue of the peptide or protein. A cleaving-reagent is then passed across the peptide or protein in the form of a spray to cleave the derivatized terminal amino acid residue from the peptide or protein. The apparatus includes a suitable support on which a peptide or protein can be disposed. The support is disposed in a reaction chamber. Pressurized sources of a suitable coupling reagent and a buffer are connected to the reaction chamber to allow exposure of the peptide or protein on the support to the coupling reagent and the buffer. A valve is also connected to the reaction chamber. The valve includes a sliding member which causes a gas and a liquid cleaving reagent to combine, whereby a spray is formed which is conducted from the valve to the reaction chamber. The spray thereby passes across the peptide or protein on the support and cleaves the derivatized terminal residue from the peptide or protein. The terminal residue is thus liberated for isolation and identification.

REFERENCES:
patent: 3892531 (1975-07-01), Gilbert
patent: 4065412 (1977-12-01), Dreyer
patent: 4603114 (1986-07-01), Hood et al.
patent: 4704256 (1987-11-01), Hood et al.
patent: 5039488 (1991-08-01), Kohr

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