Method and substance for the enhanced labelling of cellular mate

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435188, 435810, 436519, 436547, G01N 3354, C12N 996

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active

046846093

ABSTRACT:
An enhanced labelling complex for localizing markers on a prepared tissue section is disclosed. The complex includes both avidin components and biotinylated macromolecular components at a ratio preselected to provide a complex which is sufficiently large to include a large number of labels, and sufficiently small to penetrate the tissue section. The markers are localized by first introducing a biotinylated link into the tissue section and thereafter exposing the section to the labelling complex.

REFERENCES:
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4298685 (1981-11-01), Parikh et al.
Hsu et al, "A Comparative Study of the Peroxidase-Anti-Peroxidase Method and an Avidin-Biotin Complex Method for Studying Peptide . . . ", American Journal of Clinical Pathology, 75(5) (1981), pp. 734-738, Chemical Abstracts 95:20759u.
Hsu et al, "The Use of Antiavidin Antibody and Avidin-Biotin-Peroxidase Complex in Immunoperoxidase Techniques", American Journal of Clinical Pathology, 75(6) (1981), pp. 816-821, Chemical Abstract 95:128730s.
Green, "Bifunctional Reagents and the Quanternary Structure of Protein", Biochemical Journal 104(3), (1967), p. 64.
Vector Laboratories, "Lectins and Biotin-Avidin System", Vector Labs Inc., 1429 Rollins Road, Burlingame, CA 94010.

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