Method and structures employing chemically-labelled polynucleoti

Details Method and structures employing chemically-labelled polynucleoti Method and structures employing chemically-labelled polynucleoti

1989-07-20

1991-02-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435188, 435296, 435300, 435 4, 435810, 436 94, 436501, 436524, 436527, 436531, 436532, 436800, 436810, 526 27, 935 77, 935 78, 935 86, 935 87, C12Q 168, C07H 2100, G01N 33533

Patent

active

049943731

ABSTRACT:
Polynucleotide sequences in a sample of biological or nonbiological material are detected by a method involving fixing of the sequences on a solid support and forming an entity between the fixed sequences and chemically-labeled polynucleotide or oligonucleotide probes having a sequence complementary to the fixed sequence for determining the identification and/or presence of the target polynucleotide sequences. The chemical label covalently or noncovalently attached to the probe comprises a signalling moiety capable of generating a soluble signal detectable by spectrophotometric assay techniques.

REFERENCES:
patent: 3652761 (1972-03-01), Weetall
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4581333 (1986-04-01), Kourilsky et al.

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