Method and staining reagent for staining hematology sample...

Details Method and staining reagent for staining hematology sample... Method and staining reagent for staining hematology sample...

2005-02-22

2005-02-22

Wallenhorst, Maureen M. (Department: 1743)

Chemistry: analytical and immunological testing

Automated chemical analysis

With sample on test slide

C436S043000, C436S045000, C436S008000, C436S017000, C436S800000, C252S408100

Reexamination Certificate

active

06858432

ABSTRACT:
Automated staining equipment that can mix reagents is used to spray a Romanowsky stain onto slide mounted specimens which are then briefly centrifuged. The centrifugation step removes excess stain leaving only a thin film. Depending on the time of the centrifugation step, most of the organic solvent and part of the water in the stain are evaporated by airflow through the equipment. This greatly accelerates the staining reaction and preserves water soluble structures such as the granules in basophilic leukocytes. For optimal performance, this staining procedure requires a thiazin-eosin stain with about 90% to about 40% organic solvent, such as methanol, and only about 10% to about 60% water. This is a unique staining reagent in Romanowsky staining.

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