Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor
Reexamination Certificate
2006-03-14
2006-03-14
Saucier, Sandra E. (Department: 1651)
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including freezing; composition therefor
Reexamination Certificate
active
07011937
ABSTRACT:
In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The oocytes are exposed for 13–15 minutes to a solution including 1.5M PROH and 0.3M sucrose before starting the freezing process.
REFERENCES:
patent: 5985538 (1999-11-01), Stachecki
De Boucaud et al., “The use of 1,2-propanediol for cryopreservation of reclacitrant seeds: the model case of Zea mays imbibed seeds”, Cryo-Letters 9 (2) : 94-101 (1988).
Otoi et al., “The Develpment of Immature Bovine Oocytes Cryopreserved by 1,2-Propanediol”, J. Reproduction and Development 41 (4) : 361-366 (1995).
Palasz, A. T. et al.: “Cryopreservation of Mammalian Embryos and Oocytes: Recent Advances”; Biotechnology Advances, vol. 14, No. 2, pp 127-149; 1996.
Otoi Takesbige et al.: “The Development of Immature Bovine Oocytes Cryopreserved by 1,2-Propanediol”; J. Reproduction and Development, pp 361-366; 1995. Abstract only.
Otoi T. et al.: “Cryopreservation of Mature Bovine Oocytes by Vitrification in Straws”: Cryobiology, pp. 77-85; Aug. 1998, Abstract only.
J. -P. Renard et al., “Two-step freezing of two-cell rabbit embryos after partial dehydration at room temperature”, J. Reprod. Fert. 71: 573-580 (1984).
Klein Howard J.
Klein O'Neill & Singh, LLP
Medi-Cult A/S
Saucier Sandra E.
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