Method and reagents for detection of mycobacteria using superoxi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2433, 536 2432, C12Q 168, C12P 1934, C07H 2104

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055254632

ABSTRACT:
The use of the superoxide dismutase (SOD) gene system as a target for the detection and differentiation of pathogenic and non-pathogenic organisms including bacteria, fungi and protozoans is described. Also described are oligonucleotides which may be used in the form of primers and probes to amplify and detect target sequences derived from SOD genes, especially from SOD genes of pathogenic and non-pathogenic organisms.

REFERENCES:
Zhang et al. (1992) Infection & Immunity 60(6):2160-2165.
Thangaraj et al. (1990) Infect. Immun. 58(6): 1937-1942.
Colston (1990) Trop. Med. Parasitol. 41. 297-298.
Stein, et al., J. of Clinical Microbiology, 30:2462-2499 (Sep. 1992). "Detection of Coxiella burnetii by DNA Amp. Using Polymerase Chain Reaction".
Zhang, et al., Molecular Microbiology 5(2):381-391 (1991), "Genetic Analysis of Superoxide Dismutase, the 23 kD Antigen of Mycobacterium tuberculosis".
Thangaraj, et al., Nuc. Acids Res., 17:8378 (1989), "Nucleotide and deduced Amino Acid Sequence of Mycobacterium leprae manganese superoxide dismutase".

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