Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1992-06-12
1993-11-16
Ceperley, Mary E.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
436536, 436546, 436816, G01N 33542
Patent
active
052623335
ABSTRACT:
This disclosure relates to a method and reagents for determining amphetamine and d-methamphetamine in a biological fluid, such as urine. In particular, this disclosure relates to improvements in a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and d-methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross reactants such as .beta.-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH, and contacting the sample with riboflavin binding protein to reduce interference from fluorescent components in the sample. The procedure also maintains the cross reactivity of the immunoassay for tyramine at about 0.4% and for 1-methamphetamine below about 5.1% and eliminates the necessity of using controlled substances as starting materials.
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Heiman Daniel F.
Hu Hsiang-Yun Y.
Johnson Sharon A.
Abbott Laboratories
Breininger Thomas M.
Ceperley Mary E.
Steele Gregory W.
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