Method and reagents for binding chemical analytes to a substrate

Details Method and reagents for binding chemical analytes to a substrate Method and reagents for binding chemical analytes to a substrate

1993-12-13

1996-06-18

Spiegel, Carol A.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

204403, 422 8201, 422 8211, 435 75, 435962, 436151, 436528, 436543, 436807, 436822, 5483041, G01N 3353

Patent

active

055277110

ABSTRACT:
A method is provided for detecting an analyte of interest in a sample. The method involves binding the analyte to the surface of a substrate through a biotin-biotin binding protein interaction, contacting the surface-bound analyte with a quantitatively detectable analyte-binding moiety that binds thereto, measuring the quantity of detectable moiety bound to the substrate surface and deriving therefrom the quantity of analyte in solution. A preferred use for the present method is in conjunction with a piezoelectric surface transverse wave device. Novel reagents useful for carrying out the inventive method are provided as well.

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