Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-03-06
2000-06-13
Celsa, Bennett
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 74, 435 724, 435 75, 435 76, 435 78, 435810, 424450, G01N 33567
Patent
active
060748346
ABSTRACT:
The present invention provides methods and reagents for assessing lipoprotein metabolism. The methods provided are applicable for use on multiple samples in a clinical laboratory, thus obviating the need for sophisticated instrumentation, such as flow cytometry. Selected cell types in a test sample are uniformly labelled with a detectable reporter substance, so that they may later be enumerated. Pre-determined lipoprotein receptors associated with the cells of interest are labelled with a receptor-selective marker, thereby to determine the number of lipoprotein receptors per cell in the test sample. Selected cells of interest are conveniently separated from the test sample by binding thereto a specific binding substance attached to a solid support. The specific binding substance binds specifically with a characteristic determinant of the cell subset of interest. The remainder of the test sample may be washed away or discarded. The separated cells of interest may then be enumerated by measuring the amount of detectable label associated therewith, hence to determine the number of lipoprotein receptors per cell in each test sample.
REFERENCES:
patent: 5135848 (1992-08-01), Abel et al.
Abel John H.
Gottlund Kathy L.
Nau Eugene A.
Obrepalska-Bielska Barbara
Slezak Sue Ellen
Celsa Bennett
Fitzpatrick John W.
Lehigh University
Novack Michael R.
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