Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition
Patent
1996-03-29
1998-03-24
Allen, Marriane P.
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Preparation composition
436 10, 436 18, 436 63, G01N 3100
Patent
active
057312060
ABSTRACT:
A lytic reagent composition and a kit of a lytic reagent system are disclosed for the rapid isolation, identification and/or analysis of at least five subpopulations of leukocytes from a whole blood sample. The composition and system have application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. The lytic reagent composition of this invention comprises saponin and a carboxylic acid of the formula RCOOH wherein R is H or a C.sub.1-3 aliphatic hydrocarbon radial optionally substituted by one or more carbonyl and/or hydroxy groups. The kit of a lytic reagent system comprises the lytic reagent composition and a quench reagent.
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Crews Harold R.
Fischer Timothy J.
Ledis Stephen L.
Sena Ted
Allen Marriane P.
Alter Mitchell E.
Coulter Electronics Inc.
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