Method and reagent for the quantitative determination of hydroge

Details Method and reagent for the quantitative determination of hydroge Method and reagent for the quantitative determination of hydroge

1979-08-01

1981-04-07

Richman, Barry

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving uric acid

23230B, 23901, 23909, 23925, 23932, 435 11, 435 14, 435 28, C12Q 128, C12Q 154, C12Q 160, C12Q 162

Patent

active

042606799

ABSTRACT:
A method of and reagent for determining hydrogen peroxide, in which the hydrogen peroxide containing sample is treated with a colouring reagent comprising a peroxidase, a hydrogen donor and an electron or radical acceptor which react stoichiometrically with the hydrogen peroxide to produce a pigment which is then measured colorimetrically. In accordance with the invention the hydrogen donor is specifically a compound of the formula ##STR1## where R.sub.1 is CH.sub.3, C.sub.2 H.sub.5, CH.sub.2 OH, CH.sub.2 CH.sub.2 OH, CH.sub.2 CH.sub.2 NH.sub.2 or COCH.sub.3 ;

REFERENCES:
patent: 3630680 (1971-12-01), Rittesdorf et al.
patent: 3630847 (1971-12-01), Rey et al.
patent: 3642444 (1972-02-01), Guehler et al.
patent: 3979262 (1976-09-01), Hunziker
patent: 4089747 (1978-05-01), Bruschi
patent: 4098574 (1978-07-01), Dappen

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