Method and reagent for the enzymatic determination of enzyme sub

Details Method and reagent for the enzymatic determination of enzyme sub Method and reagent for the enzymatic determination of enzyme sub

1980-03-25

1985-05-14

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 10, 435 11, 435 14, 435 15, 435 16, 435 20, 435 22, 435 26, 435 28, C12Q 100, C12Q 150, C12Q 162, C12Q 128

Patent

active

045172870

ABSTRACT:
The present invention provides a reagent for the enzymatic determination of an enzyme substrate, containing a system for the determination of the substrate, which comprises at least one enzyme, at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent contains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate determination capacity of the system for the determination of this substrate.
The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substances which can react with the substrate itself or with an intermediate or end product of the indicator reaction, wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.

REFERENCES:
patent: 4000042 (1976-12-01), Adams
patent: 4241179 (1980-12-01), Madappally et al.
patent: 4242446 (1980-12-01), Madappally et al.

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