Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1978-03-31
1981-01-27
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 26, 435 27, C12Q 130, C12Q 162
Patent
active
042476305
ABSTRACT:
The invention relates to an improvement in a conventional method for the determination of uric acid involving uricase/catalase/aldehyde dehydrogenase, resulting in the formation of produced NAD(P)H as a measure of the initial uric acid present, which improvement comprises adding at least one compound selected from oxalates, malonic acid mono-lower alkyl esters, trihaloethanols, pyrazole, pyridine, substituted pyrazole and pyridine wherein the substituents are selected from lower alkyl and halogen, pyridine carboxylic acids, pyridine carboxylic acids substituted with a lower radical, pyridine carboxylic acid amides and pyridine carboxylic acid lower alkyl esters, thiourea, isobutyramide and chelate-forming complexing agents, in order to suppress disturbance-causing creep reactions in said method. Reagents containing such additive compounds are also provided.
REFERENCES:
patent: 3335069 (1967-08-01), Wachter
patent: 3956069 (1976-05-01), Allain et al.
Draeger Brigitte
Gruber Wolfgang
Hagen Alexander
Munz Eberhard
Ziegenhorn Joachim
Boehringer Mannheim GmbH
Kepplinger Esther M.
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