Method and reagent for the determination of triglycerides

Details Method and reagent for the determination of triglycerides Method and reagent for the determination of triglycerides

1980-12-05

1983-01-11

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 19, 435 26, 435194, 435810, 435832, 424 2, 436 71, C12Q 148, C12Q 144, G01N 3100, C12Q 132

Patent

active

043682618

ABSTRACT:
A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with adnosine triphosphate in the presence of glycerol kinase with the formation of glycerol-1-phosphate and adenosine diphosphate and determination of one of the latter two products. The glycerol kinase used is from Bacillus stearothermophilis and acts in combination with at least one activator selected from the group consisting of detergents, phenol derivatives and aniline derivative.

REFERENCES:
patent: 3862009 (1975-01-01), Wahlefeld et al.
patent: 4229527 (1980-10-01), Ziegenhorn et al.
patent: 4241178 (1980-12-01), Esders et al.
patent: 4245041 (1981-01-01), Denney
patent: 4246342 (1981-01-01), Misaki et al.
patent: 4259440 (1981-03-01), Gupta et al.
Comer et al., Purification and Properties of Glycerokinase from Bacillus Sternothermophilis-J. Appl. Biochem. 1(3), 1979, pp. 256-270.
Hawley, Condensed Chemical Dictionary 1974, Van Nostrand Reinhold Co., N.Y., pp. 862 and 903.
Atkinson et al., Behavior of Bacillus Sternothermophilis Grown in Different Media, J. Appl. Bacteriol., 1975, 38(3), 301-304.

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