Method and reagent for the determination of substances forming h

Details Method and reagent for the determination of substances forming h Method and reagent for the determination of substances forming h

1976-12-27

1978-07-18

Golian, Joseph M.

Textiles: fiber preparation

1951035C, 1951035R, 1951035UR, G01N 3300, G01N 3114

Patent

active

041013816

ABSTRACT:
In a conventional method for the quantitative determination of substances, particularly in serum, which, by action of an oxidase thereon, combine with oxygen to form hydrogen peroxide and wherein the hydrogen peroxide formed is converted, in the presence of methanol and catalase, to formaldehyde, the formaldehyde is reacted with hydrazone in the presence of ferric chloride, and the dye thus formed is determined photometrically, the improvement comprising using as the hydrazone, 3-methyl-2-sulfonylbenzothiazolone-hydrazone; use of this hydrazone results in the formation of a very easily soluble dye and is suited for use in automatic analyzers.

REFERENCES:
patent: 3886045 (1975-05-01), Meiattini
patent: 3979262 (1976-09-01), Hunziker

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