Method and reagent for the determination of iron

Details Method and reagent for the determination of iron Method and reagent for the determination of iron

1996-07-11

1998-06-09

Soderquist, Arlen

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

436 43, 436 73, 436 84, 436166, 436174, 436175, 422 56, 422 61, G01N 3352, G01N 3390

Patent

active

057632812

ABSTRACT:
In order to determine iron in serum by releasing the bound iron through addition of a protein denaturing agent, reduction of the released iron to Fe.sup.2+, addition of a color reagent solution and photometric measurement of the color complex that forms, a mixture of a denaturing agent containing urea or a urea derivative and a fatty alcohol polyglycol ether are added to the sample solution.

REFERENCES:
patent: 3943954 (1976-03-01), Flournoy
patent: 4579825 (1986-04-01), Siedel et al.
patent: 4961970 (1990-10-01), Siedel et al.
patent: 5149633 (1992-09-01), Vogt et al.
patent: 5219760 (1993-06-01), Herrmann et al.
S. Tajima et al. Arch. Biochem. Biophys. 1979, 198, 137-144.
H. Hoffmann et al. J. Colloid. Interfac. Sci. 1981, 80, 237-254.
R. Jha et al J. Chem. Soc., Faraday Trans. 1993, 89, 3465-3469.

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