Method and reagent for the determination of glutamate-oxalacetat

Details Method and reagent for the determination of glutamate-oxalacetat Method and reagent for the determination of glutamate-oxalacetat

1979-07-20

1981-06-02

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 26, 435805, C12Q 132, C12Q 152

Patent

active

042712653

ABSTRACT:
In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of .alpha.-ketoglutarate in a buffered solution, the .alpha.-ketoglutarate formed is reacted with .gamma.-aminobutyrate in the presence of .gamma.-aminobutyrate transaminase with formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate semialdehyde dehydrogenase to give NADPH, and the latter is measured either directly or after conversion with a tetrazolium salt and an electron carrier such as diaphorase, phenantroline methosulphate or phenazine methosulphate to a formazane dye.
A reagent suited for use with this method contains .gamma.-aminobutyrate transaminase, succinate semialdehyde dehydrogenase, .gamma.-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.

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