Method and reagent for the determination of creatine kinase MB

Details Method and reagent for the determination of creatine kinase MB Method and reagent for the determination of creatine kinase MB

1980-02-28

1982-05-18

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 8, 435 17, 435184, G01N 3354, C12Q 150, C12N 999

Patent

active

043306209

ABSTRACT:
A method for the determination of creatine kinase-MB in serum by immunological exclusion of sub-unit M and measurement of the sub-unit B for the determination of creatine kinase, the improvement comprising, for the purpose of excluding the sub-unit M, adding to the reaction mixture comprising determination reagents and the creatine kinase-containing sample, monovalent fragments obtained from antibodies against the sub-unit M by proteolytic cleavage under reducing conditions.

REFERENCES:
patent: 3932221 (1976-01-01), Pfleider
patent: 4001088 (1977-01-01), Antonik
patent: 4067775 (1978-01-01), Wurzburg et al.
patent: 4200436 (1980-04-01), Mochida
Gerhardt et al, "Creatine Kinase B-subunit Activity in Serum After Immunoinbition of M-subunit Activity", Chem. Absts., vol. 91, No. 11, p. 312 (1979) abs. No. 86102y.
Malakhov, "Interaction of Human Creatine Phosphokinase Isoenzymes With Rabbit Antibodies and Their Fab-fragments", Chem. Absts., vol. 90, No. 13 p. 416, (1979) abs. No. 101737j.
Wuerzburg, et al, "Determination of Creatine Kinase MB Activity", Chem. Absts., vol. 87, No. 7, p. 161, (1977), abs. No. 49467t.
Jockers-Wretou et al, "Quantitation of Creatine Kinase Isoenzymes in Human Tissues and Sera by an Immunological Method", Clin. Chim. Acta, vol. 58, No. 3, (1975) pp. 223-232.
Gerhardt, et al. "Creatine Kinase B-subunit Activity in Human Serum I", Clin. Chim. Acta., vol. 78, No. 1, (1977), pp. 29-41.

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