Method and reagent for the determination of creatine kinase

Details Method and reagent for the determination of creatine kinase Method and reagent for the determination of creatine kinase

1980-02-28

1981-08-25

Castel, Benoit

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 17, 435810, C12Q 166

Patent

active

042860571

ABSTRACT:
Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.

REFERENCES:
patent: 3575811 (1971-04-01), Chappelle et al.
patent: 4080265 (1978-03-01), Antonik
Lundin A. et al.; Clinica Chimica Acta, vol. 87, pp. 199-209, (1978).
Gates B. J. et al.; Archives of Biochemistry and Biophysics; vol. 169, pp. 616-621, (1975).
Lee R. T. et al.; Archives of Biochemistry and Biophysics, vol. 141, pp. 38-52; (1970).

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