Method and reagent for the determination of .beta.-lipoprotein

Details Method and reagent for the determination of .beta.-lipoprotein Method and reagent for the determination of .beta.-lipoprotein

1983-05-04

1984-12-04

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

436 13, 436 15, 436 17, 436 71, 436177, 436175, 435810, 435 11, G01N 3368, G01N 3392, C12Q 144, C12Q 160

Patent

active

044865317

ABSTRACT:
The present invention provides a process for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids by precipitation with polyanions and divalent cations, wherein the precipitation is carried out in the presence of a complex former at a pH value of from 7 to 9.
The present invention also provides a reagent for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids, comprising a polyanion, a salt of a divalent metal, a complex former and a substance buffering at pH 7 to 9.

REFERENCES:
patent: 4039285 (1977-08-01), Teipel
patent: 4066508 (1978-01-01), Rauscher et al.
patent: 4110077 (1978-08-01), Klein et al.
patent: 4309364 (1982-01-01), Bentzen et al.
Kim et al., "Nature of the Interaction of Dextran Sulfate with High and Low Density Lipoproteins in the Presence of Ca.sup.2 +", Journal of Biological Chemistry, (10-1979), pp. 9621-9626.
Allen et al., "An Enzymatic and Centrifugal Method for Estimating High-Density Lipoprotein Cholesterol", Clinical Chemistry, vol. 25, (2), (1979), pp. 325-327.
Hawley, The Condensed Chemical Dictionary, Van Nostrand Reinhold Co., N.Y., (1974), pp. 190, 191, 233 and 516.

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