Method and reagent for the determination of antithrombin III

Details Method and reagent for the determination of antithrombin III Method and reagent for the determination of antithrombin III

1993-09-15

1994-06-14

Johnston, Jill A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

436 69, C12Q 156

Patent

active

053209451

ABSTRACT:
In order to determine antithrombin III in body fluids by reacting the sample with thrombin and a chromogenic substrate which forms a colour by the action of thrombin and measurement of the colour formed, the reaction is carried out in the presence of denaturing agents or of the tetrapeptide Gly-Pro-Arg-Pro and an oligopeptide is used as the substrate which has a sensitivity to thrombin which is a factor of 5 to 100-fold lower compared With Tos-Gly-Pro-Arg-pNA

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Analytical Biochemistry (1985) vol. 144, pp. 165-171.

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