Method and reagent for the assay of hydroperoxide

Details Method and reagent for the assay of hydroperoxide Method and reagent for the assay of hydroperoxide

1977-11-11

1979-03-06

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

1951035R, 1951035C, 252408, G01N 3316

Patent

active

041430808

ABSTRACT:
In a method for detection and/or assay of hydroperoxides (or, indirectly, of substances, such as cholesterol or glucose, which react to liberate hydroperoxides) using an assay reagent comprising a water-soluble iodide, a redox catalyst and a stabilizer, an improvement is provided wherein when the determination is made in the presence of protein, the redox catalyst concentration in the reagent is 5-500 .mu.M and the stabilizer concentration in the reagent is 5-100 mg/l. A corresponding improvement in the assay reagent is also provided.

REFERENCES:
patent: 3016292 (1962-01-01), Bauer et al.
patent: 3266868 (1966-08-01), Marvill
patent: 3335069 (1967-08-01), Wachter
patent: 3404069 (1968-10-01), Ware
patent: 3654180 (1972-04-01), Bauer
patent: 4040908 (1977-08-01), Clark, Jr.
Malmstadt et al.; "A New Automatic Spectrophotometric Rate Method for Selective Determination of Glucose in Serum, Plasma or Blood"; Analytical Chemistry, vol. 34, No. 4, Apr. 1962, pp. 452-455.

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