Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1989-03-06
1995-09-12
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2534, 536126, 501 39, C07H 1512, C07H 1700
Patent
active
054497698
ABSTRACT:
A composition suitable for sulfurizing an organophosphite. The reagent comprises a solution of sulfur and a tertiary amine, in a suitable solvent such as carbon disulfide. The tertiary amine is preferably an optionally substituted trialkyl amine, for example triethylamine or diisopropylamine. A method is also provided in which phosphites or phosphonous esters are sulfurized to their corresponding phosphorothioates or phosphonothioates, respectively, using compositions of the present invention. This method has particular application to produce internucleotide phosphorothioate or phosphonothioate bonds in a nucleotide multimer.
REFERENCES:
patent: 2536647 (1951-01-01), Kosolapoff
patent: 2647140 (1953-07-01), Jonas
patent: 2907787 (1959-10-01), Hoffman et al.
patent: 3162570 (1964-12-01), Wilson
patent: 3294874 (1966-12-01), Schrader
patent: 3296221 (1967-01-01), Stanley
patent: 3687808 (1972-08-01), Merigan et al.
patent: 4189453 (1980-02-01), Zinke et al.
Connolly et al Biochemistry vol. 23 p. 3443 (1984).
Hata, T. & Sekine, M. Oxidation of Nucleoside Phosphites By Means of 2,2' Dipyridyl Disulflide Via Nucleoside Silylphosphite Intermediates. Tetrahedron Letters No. 45, pp. 3943-3946 (1974).
Chen, J. & Benkovic, S. Synthesis and Separation of Diastereomers of Deoxynucleoside 5' 0-(1-Thio) Triphosphiates, Nucleic Acids Research 11, 3737-3751 (Jun. 11, 1983).
Lamande, L., et al.--Synthesis of a Triethylammonium Salt of a Spirophosphorane Containing a P-SH Bond Society of Chemical Communications 49, 2314-2317 (1984).
Brown Johnnie R.
Gen-Probe Incorporated
Wilson James O.
LandOfFree
Method and reagent for sulfurization of organophosphorous compou does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and reagent for sulfurization of organophosphorous compou, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and reagent for sulfurization of organophosphorous compou will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-406168