Method and reagent for quantitative analysis of l-lysine in prot

Details Method and reagent for quantitative analysis of l-lysine in prot Method and reagent for quantitative analysis of l-lysine in prot

1975-04-30

1976-11-30

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

23230B, 260279R, G01N 3302, C07D21900

Patent

active

039946886

ABSTRACT:
A test sample containing L-lysine in proteinaceous substances is reacted with a .gamma.-formyl-pyridine reagent of the general formula ##SPC1##
Wherein R.sup.1 and R.sup.2 are each aliphatic carbon groups having not more than 4 carbon atoms or forming with two adjacent carbon atoms in the pyridine ring aromatic rings, in order to form the corresponding Schiff base, and the amount of reacted pyridine reagent is determined.

REFERENCES:
patent: 3539574 (1970-11-01), Sheehan et al.
patent: 3689221 (1972-09-01), Udenfriend
rapaport et al., J. Am. Chem. Soc., v. 94(9), pp. 3160-3167 (1972).

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