Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1990-08-28
1992-02-04
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, C12Q 126, C12Q 132
Patent
active
050859897
ABSTRACT:
A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.
REFERENCES:
patent: 3791933 (1974-02-01), Moyer et al.
patent: 4816394 (1989-03-01), Ushizawa et al.
Christiansen et al., Chemical Abstracts, 103:84307m, 9/16/85, p. 303.
Chemical Abstracts, vol. 107, No. 13, Sept. 28, 1987, p. 306 abstract No. 111955n.
Chemical Abstract, vol. 76, No. 13, Mar. 27, 1972, p. 138 abstract No. 69430b.
Comprehensive Biochemistry, vol. 13, 3rd Ed., pp. 80,81 Florkin et al.
Akimoto Toshiyuki
Hirata Miyoshi
Nagasaki Senkichi
Shigihara Takae
Ushizawa Koji
Daiichi Pure Chemicals Co. Ltd.
Naff David M.
LandOfFree
Method and reagent for quantitative analysis of 3-oxo-5.beta.-st does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and reagent for quantitative analysis of 3-oxo-5.beta.-st, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and reagent for quantitative analysis of 3-oxo-5.beta.-st will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-347582