Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-07-13
1997-12-02
Richter, Johann
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
536 41, C12Q 134, C07G 300
Patent
active
056934807
ABSTRACT:
According to this invention are provided a method for determining an exo-type saccharide hydrolase activity which comprises adding to a sample a .beta.-maltoside derivative represented by the general formula ##STR1## wherein R is a chromophoric organic group, as a substrate, conducting reaction in the presence of a coupled enzyme, and optically determining the substance liberated, and a method for differentially determining glucoamylase and .alpha.-glucosidase activities which comprises adding, to a sample, the above .beta.-maltoside derivative as the first substrate and a coupled enzyme, and an .alpha.-glucoside derivative represented by the general formula ##STR2## wherein R' is a chromophoric organic group, as the second substrate, subjecting the mixtures to separate enzymatic reactions, and then optically quantitatively determine the substances liberated.
REFERENCES:
Agric. Biol. Chem. 47 (8), 1741-1746 Seiya et al. (1983).
Imai Yasuhiko
Suzuki Masaru
Tokutake Shoichi
Yamaji Nobuyuki
Kikkoman Corporation
Richter Johann
Sackey Ebenezer
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