Method and reagent for determining LD-1 isoenzyme

Details Method and reagent for determining LD-1 isoenzyme Method and reagent for determining LD-1 isoenzyme

1989-11-06

1992-10-27

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435184, 436174, 436175, 436176, 436825, 252 95, 252100, 2521861, C12Q 132, C12N 999

Patent

active

051588730

ABSTRACT:
A rapid and specific method and reagent for determining LD-1 isoenzyme in biological fluids by incorporating a chaotropic agent, such as sodium perchlorate, into an LDH assay reagent system. The chaotropic agent produces an immediate inactivation of the LDH isoenzymes containing one or more `M` type subunits while the LTD-1 isoenzyme remains stable and is simultaneously measured.

REFERENCES:
patent: 4145406 (1979-03-01), Schick et al.
patent: 4250255 (1981-02-01), Sanford
patent: 4258131 (1981-03-01), Takagahara et al.
Somero et al., Arch. Biochem Biophys., vol. 181, (1977) pp. 438-446.
Tietz, N. (ed), Textbook of Clinical Chemistry, 1986, pp. 148-149.
Forman et al, Journal of Biological Chemistry, vol. 252, No. 10 (1977) pp. 3379-3381.
Wilkinson, The Principles and Practice of Diagnostic Enzymology, (1976), pp. 46-54.
Isomune-LD.RTM., Roche Diagnostic Systems, Nutley, N. J., Package Insert Apr., 1986.

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