Method and reagent for determining inorganic phosphate in biolog

Details Method and reagent for determining inorganic phosphate in biolog Method and reagent for determining inorganic phosphate in biolog

1982-07-22

1984-05-08

Marantz, Sidney

Chemistry: analytical and immunological testing

Phosphorus containing

Of inorganic phosphorus compound in body fluid

436 19, 436175, G01N 3352, G01N 3384, G01N 3396

Patent

active

044475446

ABSTRACT:
An aqueous reagent for determining inorganic phosphate in a protein-containing liquid biological sample comprises a molybdate salt, an acid capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a ferric salt in an amount sufficient to inhibit turbidity in the sample, and a nonionic surfactant reagent in an amount sufficient to further inhibit turbidity in the sample, the molybdate salt and the acid being present in amounts sufficient to form an amount of molybdic acid sufficient to produce a detectible color change when complexed with phosphate in the sample.

REFERENCES:
patent: 3771961 (1973-11-01), Denney
patent: 3795484 (1974-03-01), Daly et al.
patent: 3853469 (1974-12-01), Morin et al.
patent: 3874853 (1975-04-01), Byrnes
patent: 3920580 (1975-11-01), Mast
patent: 3953359 (1976-04-01), Gindler
patent: 4009004 (1977-02-01), Hutchinson
patent: 4220451 (1980-09-01), Stefanchik
Chemical Abstracts, 94:188223, (1981).
John A. Daly, et al., "Direct Method for Determining Inorganic Phosphate in Serum with the `CentrifiChem`", Clinical Chemistry, vol. 18, (1972), pp. 263-265.
Baadenhuijsen, et al., "Continuous-Flow Determination of Serum Inorganic Phosphate with a Single Reagent-The Vanadomolybdate Method Re-Evaluated", Clinical Chemistry, vol. 23, (1977), pp. 1275-1280.
Worthington, "Inorganic Phosphorus Reagent Set", Worthington Diagnostics, pp. 69-71, (1978).
Stanbio Laboratory Inc., "Fast (1 Min) Phosphorus Test", Set No. 0830, (1981).

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