Method and reagent for determining biologically active heparin i

Details Method and reagent for determining biologically active heparin i Method and reagent for determining biologically active heparin i

1979-02-21

1980-11-18

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 23, C12Q 138, C12Q 156

Patent

active

042346827

ABSTRACT:
A method for determining biologically active heparin comprising adding, in the absence of exogenous antithrombin III, a proteolytic enzyme selected from the group consisting of thrombin and factor Xa and a chromogen substrate for the enzyme to a heparin sample, measuring the dye released from the chromogen substrate and thus determining the amount of heparin present.

REFERENCES:
patent: 3884896 (1975-05-01), Blomback et al.
patent: 4061625 (1977-12-01), Ekenstam et al.
patent: 4067777 (1978-01-01), Innerfield et al.
patent: 4070245 (1978-01-01), Svendsen
patent: 4106990 (1978-08-01), Karges et al.
patent: 4162941 (1979-07-01), Aurell et al.
Witt, J. Clin. Chem. Clin. Biochem., 15, 239-244 (1977).
Teien et al, Chemical Abstracts 86:27402v (1976), p. 164.

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