Method and reagent for determination of an analyte via enzymatic

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

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435 4, 435 14, 435 28, C12Q 158

Patent

active

049295454

ABSTRACT:
A composition useful in determining analytes, such as glucose or cholesterol, is disclosed. The composition contains an enzyme which specifically reacts with the analyte to be determined, a soluble ferricyanide compound which is reducible to form a ferrocyanide, a soluble ferric compound which provides ferric ions for reacting with ferrocyanide to form a reaction product, and a buffer which does not prevent formation of this reaction product. The composition has a pH of from about 3.0 to about 6.0.

REFERENCES:
patent: 4576913 (1986-03-01), Adachi et al.

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