Method and reagent for detecting peroxidase or hydrogen peroxide

Details Method and reagent for detecting peroxidase or hydrogen peroxide Method and reagent for detecting peroxidase or hydrogen peroxide

1996-07-18

1998-07-14

Leary, Louise

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435968, 423582, 423584, 564300, 436173, C12Q 128, C12Q 126, G01N 3353, G01N 2400

Patent

active

057802572

ABSTRACT:
Method for detecting peroxidase or hydrogen peroxide with high sensitivity. Both peroxidase and hydrogen peroxide are prepared such that one of them is overabundant to the other. Phenoxy radicals are produced from a p-substituted phenol compound by the action of peroxidase in the presence of hydrogen peroxide. The free radicals are trapped by a hydroxy amine compound, and stable radical species are produced. Electron spin resonances of the stable radical species are measured.

REFERENCES:
patent: 4828983 (1989-05-01), McClune
patent: 5372931 (1994-12-01), Friedman et al.
Fischer et al., "Direct Electron Spin Resonance Detection of Free Radical Intermediates During The Peroxidase Catalyzed Oxidation of Phenacetin Metabolites," Chemico-Biological Interactions 1986, 60, 115-127, Nov. 1986.
Ross et al., "The Generation and Subsequent Fate of Glutathonyl Radicals in Biological Systems," J. Biol. Chem. 1985, 260, 15028-15032, Dec. 5, 1985.
Thorpe, Gary H. G., et al., "Enhancement of the Horseradish Peroxidate-Catalyzed Chemiluminescent Oxidation of Cyclic Diacyl Hydrazides by 6-Hydroxybenzothiazoles", Analytical Biochemistry 145, 96-100 (1985), pp. 96-100.
Klebanoff, S. J., "An Effect of Thyroxine and Related Compounds on the Oxidation of Certain Hydrogen Donors by the Peroxidase System", Journal of Biological Chemistry, vol. 234, No. 9, Sep. 1959, pp. 2437-2442.
Toshiyuki Ohnishi et al., "One-Electron-Transfer Reactions In Biochemical Systems", Biochim. Biophys. Acta, 172 (1969) pp. 357-369.

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