Method and reagent for detecting a ligand in a sample

Details Method and reagent for detecting a ligand in a sample Method and reagent for detecting a ligand in a sample

1996-09-06

1998-10-13

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 21, 435183, 536 221, 536 2532, 530350, C12Q 168, C12P 1934, C07H 1900, C07K 100

Patent

active

058210610

ABSTRACT:
A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.

REFERENCES:
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patent: 5118605 (1992-06-01), Urdea
Boyer, P.D., ed. The Enzymes, 3rd Edition, "Mammalian Alkaline Phosphatases" (1971) vol. 4, 417-447.
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F.M. Hulett, Stuckmann, K., Spencer, D.B., Sanopoulou, T., Purification and Characterization of the Secreted Alkaline Phosphatase of Bacillus licheniformis MC14: Identification of a Possible Precursor (1986) Journal of General Microbiology 132, 2387-2395.
Leary et al. "Rapid and sensitive colorimetric method for visualizing biotin-labeled probes hybridized to DNA or RNA immobilized on nitrocellulose:Bio-blots" Proc. Natl. Acad. USA, vol. 80, pp. 4045-4049, Jul. 1983.
Jablonski et al. "Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes" Nucleic Acids Research, vol. 14, No: 15, pp. 6115-6128, 1986.
(Nomoto et al.) Agricultural and Biological Chemistry, vol. 52, No. 7, Jul., 1988, pp. 1643-1647, XP002022417, "Purification and Characterization of Extracellular Alkaline Phosphatase from an Alkalophilic Bacterium".

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