Method and reagent for bioluminiscence

Details Method and reagent for bioluminiscence Method and reagent for bioluminiscence

1979-05-30

1981-01-20

Therkorn, Ernest G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435184, C12Q 166

Patent

active

042463408

ABSTRACT:
An improvement in the process for the determination of the concentration of adenosinetriphosphate (ATP) in ATP-converting reactions wherein the concentration is a function of the intensity of light emitted when a sample to be assayed is contacted with a bioluminescent reagent based on D-luciferin, luciferase and magnesium ions or certain other metal ions, the emitted light resulting from ATP and D-luciferin becoming bound to the luciferase, comprises the use of one or more competitive inhibitors such as a D-luciferin analogue, or analogues.

REFERENCES:
patent: 3575811 (1971-04-01), Chappelle
patent: 3575812 (1971-04-01), Chappelle
patent: 3616253 (1971-10-01), D'Eustachio
patent: 3929586 (1975-12-01), Forgione
patent: 3933592 (1976-01-01), Clendenning
patent: 4001088 (1977-01-01), Antonik
patent: 4080265 (1978-03-01), Antonik
"Immobilized Firefly Luciferase," Lee, Analytical Biochemistry 80 (1977) pp. 496-501.

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