Method and reagent compositions for determining alpha amylase us

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 22, 435810, C12Q 134, C12Q 140

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active

048186929

ABSTRACT:
The present invention provides compounds of the general formula: ##STR1## in which R and R.sub.1, independently of one another, each represent a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms or a phenyl radical or R and R.sub.1 together also form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, R.sub.2 represents an oligoglucoside residue containing 2 to 7 glucose units and X is a hydrogen atom or an optically-determinable residue.
The present invention also provides a process for the preparation of these compounds.

REFERENCES:
patent: 4102747 (1978-07-01), Driscoll et al.
patent: 4233403 (1980-11-01), Menson et al.
patent: 4550077 (1981-07-01), Woodbridge
patent: 4622295 (1986-11-01), Ikenaka et al.
patent: 4649108 (1987-03-01), Blair
Laeseche et al., Liebigs Ann. Chem., 11, 1910-1919 (1983).
Green, in Whistler & Wolferen (eds). Methods in Carbohydrate Chemistry, vol. 2 (Academic Press, N.Y. 1963).
Matsui et al., "Research of Reactivity and Hydrolysis Type on Various Substrates for alpha Amylase Assay by HPLC", Japan Clinical Chemistry Association Summer Seminar (Jul. 1982).
Marshall: Anal. Biochem. 85: 541-549 (1978).
Marshall et al.: Clin. Chim. Acta 76: 277-283 (1977).
Biomedix, "Amylase U.V. Delta Test Assay" (Becton Dickinson, 1980).

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