Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1995-05-16
1997-06-17
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 18, 435 15, 435 4, 435 724, 435 4051, 436 63, 436 74, 436 17, 436 16, C12Q 128, C12Q 134, C12N 500, G01N 3348
Patent
active
056396309
ABSTRACT:
The present invention provides an improved reagent composition and method to perform white blood cell differential counting and subpopulation analysis using both fresh and aged blood samples with accuracy and precision. The invention is particularly applicable for the analysis of aged blood samples that have been stored at room temperature for over a day, thereby allowing accurate and useful information to be obtained from samples that are normally considered to be suboptimal. The improved reagent composition and method are particularly related to the peroxidase method of white blood cell differential determinations. One aspect of the invention includes an improved aqueous reagent composition for carrying out the peroxidase method of differential counting. Another aspect includes the use of a rinse cycle and rinse solution devoid of hemolytic surfactant to alleviate the adverse effects of rinse carryover and to streamline and economize the analytical process, particularly when the analyses are performed on automated hematology analyzers and flow cytometry systems. The composition and method of the invention provide clinically useful data for the differential analysis of whole blood samples.
REFERENCES:
patent: 3741875 (1973-06-01), Ansley et al.
patent: 4099917 (1978-07-01), Kim
patent: 4801549 (1989-01-01), Cremins et al.
patent: 4978624 (1990-12-01), Cremins et al.
patent: 5116539 (1992-05-01), Hamaguchi et al.
patent: 5116734 (1992-05-01), Higgs et al.
patent: 5128265 (1992-07-01), Meiattini
patent: 5232857 (1993-08-01), Lefevre et al.
patent: 5262302 (1993-11-01), Russell
patent: 5264369 (1993-11-01), Saka et al.
patent: 5389549 (1995-02-01), Hamaguchi et al.
patent: 5426029 (1995-06-01), Rittershaus
patent: 5518928 (1996-05-01), Cremins et al.
patent: 5525461 (1996-06-01), Rittershaus
patent: 5538893 (1996-07-01), Sakata et al.
Technicon.RTM. Technical Publication No. UAB-3515-00, Product Labeling Technicon H6000 System (90/h), Section I, pp. 1-8, Apr., 1993.
Technicon.RTM. Addendum No. TK81-443-20, Technical Publication No. UA81-443B00, Addendum to vol. II of the Product Labeling for the Technicon H6000 System.
Cremins John F.
Malin Michael J.
Shapiro Phyllis
Bayer Corporation
Kight John
Leary Louise
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