Method and reactive composition suitable for the colorimetric de

Details Method and reactive composition suitable for the colorimetric de Method and reactive composition suitable for the colorimetric de

1986-09-05

1987-10-27

Bashore, S. Leon

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

436 84, 422 61, G01N 3348, G01N 3352

Patent

active

047030151

ABSTRACT:
A colorimetric reagent formulation is disclosed for the determination of the ferric iron content of serum. The formulation consists of two aqueous solutions. One solution, acting as chromogenic reagent, consists of a buffer, a soluble salt of an alkali or alkaline earth metal, chromazurol S in acidic form, and either a cationic or nonionic surfactant. The other solution, acting as masking reagent, consists of a complexing agent and if desired the same components of the chromogenic reagent, which are able to prevent the formation of the complex between the chromazurol S, the surfactant and the iron. The complexing agent consists of a polycarboxylic organic acid or salt thereof.

REFERENCES:
patent: 4407962 (1983-10-01), Tabacco et al.
Kuban et al, "Comparison of the Reactions of Ferric Ions with Chromazurol S, Eriochromazurol B and Eriochromcyanine R in the Presence of Surface Active Substances . . . ", Collect. Czech. Chem. Commun., 45(10), 2656-69 (1980).
Colour Index, 3rd Ed. (1971) p. 4408.

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