Method and reaction kit for agglutination detection

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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422 58, 422 61, 422 73, 422102, 436165, 436520, 436539, G01N 3353

Patent

active

051889684

ABSTRACT:
A reaction kit is provided with a reaction zone of suitable cross-sectional area to aspirate a sample by capillarity and a transparent plate with a flat surface in at least part of the reaction zone, and a supporting base to incline the reaction zone at a specified angle. The reaction zone is normally a space formed by two opposite transparent plates and spacers inserted between these two transparent plates. A supporting base may be molded separately, the transparent plates and spacers being set on the base at the time of measurement, or alternatively the base may be molded integrally with at least one of the plates defining the reaction zone. Further, substances with specificity such as antigens or antibodies may be coated on a surface in the reaction zone. The occurrence or non-occurrence of an agglutination reaction may be determined by introducing a particle suspension containing particles with specific bonding properties and a substance with specific bonding properties into the reaction zone by means of capillarity, leaving the apparatus for a predetermined time to carry out the reaction, and thereafter observing the particle distribution pattern.

REFERENCES:
patent: 4303616 (1981-12-01), Kano et al.
patent: 4436827 (1984-03-01), Tamagawa
patent: 4591570 (1986-05-01), Chang
patent: 4596695 (1986-06-01), Cottingham
patent: 4756884 (1988-07-01), Hillman et al.
patent: 5066465 (1991-11-01), Kano et al.

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