Method and probes for detecting nucleoside transporter and metho

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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536 174, 536 185, 536 2762, 436501, A61K 3170, C07H 19167, C07H 1916, G01N 33566

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RE0359041

ABSTRACT:
The present invention is directed to compounds capable of binding to the es nucleoside transporter of animal cells having the general formula: ##STR1## wherein n is 1-12; E is H, halogen, NH.sub.2, OH, OCH.sub.3, O(CH.sub.2).sub.n CH.sub.3 (where n is 1 to 12), SH, SR (where R is CH.sub.3 or (CH.sub.2).sub.n CH.sub.3 and n is 1 to 12); A is HN, S, O, Se; X is N or C; Y is N or C; Z is N or C; R.sub.1 is H or acyl; R.sub.2 is C1 to C20 substituted or unsubstituted alkyl or heteroalkyl; substituted or unsubstituted aliphatic carbocycle or heterocycle; substituted or unsubstituted arene or heteroarene, aryl or substituted aryl; heteroaryl or substituted heteroaryl; R.sub.3 is H, halogen, NO.sub.2, N.sub.3, SH, SR (where R is CH.sub.3 or (CH.sub.2).sub.n CH.sub.3 and n is 1 to 12) when Y is C and R.sub.3 is absent when Y R.sub.5 is H, OH, halogen, N.sub.3, acetal, hemiacetal; and is N.

REFERENCES:
F. Agbanyo et al., "5'-S(2-Aminoethyl)-N6-(4-Nitrobenzyl)-5'thioadenosine (SAENTA), A Novel Ligand With High Affinity for Polypeptides Associated With Nucleoside Transport", Biochem. J. 270: 605-614, Sep. 1990.
J.S. Wiley et al., "Cytosine Arabinoside Influx and Nucleoside Transport Sites in Acute Leukemia", J. Clin. Invest. 69: 479-489, Jan. 1982.
Gati, W. P. & Paterson, A.R.P. "Nucleoside Transport", Red Blood Cell Membranes, Agre, P. & Parker, J.C. (eds.) Marcel Dekker, New York (1989), pp. 635-661.
Wiley, J.S. et al., "Cyosine Arabinoside in the Treatment of T-Cell Acute Lymphoblastic Leukemia", Australia & New Zealand Journal of Medicine, 1987, 17:379-386.
Wiley, J.S. et al. "Nucleoside Transport in Acute Leukaemia and Lymphoma: Close Relation to Proliferative Rate", British Journal of Haematology, 1989, 71:203-207. high-affinity nucleoside transproter probe", Biochemistry and Cell Biology, 1987, 65:467-473.
Shohami, E. & Koren, R., "S-(N-Dansylaminoethyl)-6-mercaptoguanosine as a Fluorescent Probe for the Uridine Transport System in Human Erythrocytes", Biochemical Journal, 1979, 178:271-277.
Paterson, A.R.P. et al. "Inhibiton by Nitrobenzylthioinosine of Adenosine Uptake by Asynchronous HeLa Cells", Molecular Pharmacology 1977, 13:1147-1158.
Gati, W.P. et al., "Interaction of 2'-Halogeno-2'-deoxyuridines with the Human Erythrocyte Nucleoside Transport Mechanism", Molecular Pharmacology, 1983, 23:146-152.
Steck, T.L. & Kant, J.A. "Preparation of Impermeable Ghosts and Inside-out Vesicles From Human Erythrocyte Membranes", Methods in Enzymology, vol. 31, (Fleischer, S. & Packer, S. eds) Academic Press, New York, 1974 pp. 172-180.
Paul. B. et al., "Inhibitors of Nucleoside Transport, A Structure-Activity Study Using Human Erythrocytes", Journal of Medicinal Chemistry, 1975, 18:968-973.
Agbanyo, F.R. et al., "External Location of Sites on Pig Erythrocyte Membranes that Bind Nitrobenzylthioinosine", Molecular Pharmacology, 1988, 33:332-337.
Pande, S.V., "Liquid Scintillation Counting of Aqueous Samples Using Triton-Containing Scintillants", Analytical Biochemistry, 1976, 74:25-34.
Smith, P.K. et al., "Measurement of Protein Using Bicinchoninic Acid", Analytical Biochemistry, 1985, 150:76-85.
Bradley, et al., "Cell Lines Derived From a Human Myelomonocytic Leukaemia", British Journal of Haematology, 1982, 51:595-604.
Jamieson, G.P. et al., "Transport and Metabolism of 1-.beta.-D-Arabinofuranosylcyosine in Human Ovarian Adenocarcinoma Cells", Cancer Research, 1989, 49:309-313.
Wiley et al., "Cytosine Arabinoside Influx and Nucleoside Transport Sites in Acute Leukemia", Journal of Clinical Investigation, 1982, 69:479-489. Nucleoside Transporter-Associated Binding Sites on S49 Mouse Lymphoma Cells", Molecular Pharmacology, 1989, 36: 134-141.
H. Hibasami et al. "Studies of Inhibition of Rat Spermidine Synthetase . . . " Biochem. J. 187: 419-428 (1980).
K. Samejima et al. "Achin of Decarboxylated S-Adenosjmethimine . . . " Arch. Biochem. Biophys. 201(1) 241-246 (Apr. 1980).

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