Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1994-12-02
1997-02-04
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 6, 435 912, 436 8, 436 94, 208 20, 208 21, C12Q 168, C12P 1934, C10G 7336
Patent
active
055996604
ABSTRACT:
A method and preparation for the storage and delivery of purified reagents is disclosed. In one aspect, the preparation comprises an amount of a first wax carrier, the first wax carrier having a first melting point, and an amount of a first reagent, wherein first reagent is a substantially purified preparation of at least one biological or chemical reagent. The first wax carrier and the first reagent are combined to form a solid first reagent portion. The solid mixture is combined with a second reagent portion comprised of at least one biological or chemical reagent in an inactive form.
REFERENCES:
patent: 3897308 (1975-07-01), Li et al.
patent: 4935339 (1990-06-01), Zahradnik
patent: 4940665 (1990-07-01), Iijima et al.
patent: 5017256 (1991-05-01), Ohtomo et al.
patent: 5098893 (1992-03-01), Franks et al.
patent: 5310885 (1994-05-01), Maier et al.
patent: 5411876 (1995-05-01), Bloch et al.
patent: 5413924 (1995-05-01), Kosak et al.
Okahata et al., J. Am. Chem. Soc. 114, 8299-8300 (1992).
Ramanujam et al., Bio Techniques 14(3), 470-474 (1993).
Bassam, et al., "Automated `Hot Start` PCR Using Mineral Oil and Paraffin Wax," Biotechniques 14:31-33, 1993.
Chou, et al., "Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications," Nucl. Acids Res. 20:1717-1723, 1992.
D'Aquila, et al., "Maximizing sensitivity and specificity of PCR by preamplification heating," Nucl. Acids Res. 19:3749, 1991.
Darnell, et al., Molecular Cell Biology, pp. 63-66, 1986, published: Scientific American Books, NY, NY.
Earley, et al., "Efficient DNA sequencing on microtiter plates using dried reagents and Bst DNA polymerase," DNA Sequence--J. DNA Sequencing and Mapping 4:79-85, 1993.
Erlich, et al., "Recent Advances in the Polymerase Chain Reaction, " Science 252:1643-1651, 1991.
Goelz, et al., "Purification of DNA From Formaldehyde Fixed and Paraffin Embedded Human Tissue," Biochem. and Biophys. Res. Commun. 130[1]:118-126, 1985.
Heller, et al., "An Efficient Method for the Extraction of DNA from Formalin-Fixed, Paraffin-Embedded Tissue by Sonication," Biotechniques 11[3]:372-377, 1991.
Impraim, et al., "Analysis of DNA Extracted from Formalin-Fixed, Paraffin-Embedded Tissues by Enzymatic Amplification and Hybridization with Sequence-Specific Oligonucleotides," Biochem. and Biophys. Res. Commun. 142[3]:710-716, 1987.
Kaijalainen, et al., "An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose," Nucl. Acids Res. 21:2959-2960, 1993.
Katzman, "Use of Oil Overlays in `Oil-Free` PCR Technology," Biotechniques 4:36-40, 1993.
Kwok, et al., "Avoiding false positives with PCR," Nature 339:237-238, 1989.
Lagerstrom, et al.; "Capture PCR: Efficient Amplification of DNA Fragments Adjacent to a Known Sequence in Human and YAC DNA," PCR Meth. and Applic. 1:111-119, 1991.
Lai-Goldman, et al., "Detection of Human Immunodeficiency Virus (HIV) Infection in Formalin-Fixed, Paraffin-Embedded Tissues by DNA Amplication," Nucl. Acids Res. 16[16]:8191, 1988.
Limitekk Advertisement, Biotechniques Apr. 1994.
Mullis, "The Polymerase Chain Reaction in an Anemic Mode: How to Avoid Cold Oligodeoxyribonuclear Fusion," PCR Methods and Applications 1:1-4, 1991.
Parik, et al., "A Manifold Support for Molecular Gentic Reactions," Anal. Biochem. 211:144-150, 1993.
Perkin-Elmer Corporation - Pamphlet on AmpliWax PCR Gems - Mar. 1992.
Rey, "Glimpses into the Fundamental Aspects of Freeze-drying," Devel. Biol. Stand. 36:19-27, 1977.
Ruano, et al., "Heat-Soaked PCR: An Efficient Method for DNA Amplification with Applications to Forensic Analysis," Biotechniques 13:266-274, 1992.
Saiki, et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase," Science 239:487-491, 1988.
Shi, et al., "Direct Reverse Transcription--Polymerase Chain Reaction from Whole Blood Without RNA Extraction," GATA 9(5-6):149-150, 1992.
Smith, et al., "Extraction of Cellular DNA from Human Cells and Tissues Fixed in Ethanol," Anal. Biochem. 160:135-138, 1987.
Stanta, et al., "RNA Extracted from Paraffin-Embedded Human Tissues is Amenable to Analysis by PCR Amplification," Biotechniques 11[3]:304-308, 1991.
Takezawa, et al., "Cell Culture on a Thermo-responsive Polymer Surface," Bio/Technol. 8:854-856, 1990.
Veres, et al., "The Molecular Basis of the Sparse Fur Mouse Mutation," Science 237:415-417, 1987.
Wainwright, et al., "Parafin Beads Can Replace Mineral Oil as an Evaporation Barrier in PCR," Biotechniques 14:35-36, 1993.
Watson, et al., "Molecular Biology of the Gene," pp. 734-735, 1976.
Burdick Brent A.
Landegren Ulf D.
Ramanujam Rama P.
Sevigny Pierre
Horlick Kenneth R.
Pharmacia Biotech Inc.
LandOfFree
Method and preparation for sequential delivery of wax-embedded, does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and preparation for sequential delivery of wax-embedded, , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and preparation for sequential delivery of wax-embedded, will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-677355