Method and polynucleotides for determining translational...

Details Method and polynucleotides for determining translational... Method and polynucleotides for determining translational...

2005-01-25

2005-01-25

McKelvey, Terry (Department: 1636)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S243000, C435S320100, C435S419000, C536S023200, C536S023400

Reexamination Certificate

active

06846671

ABSTRACT:
A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method comprises introducing into the cell a synthetic construct comprising a reporter polynucleotide fused in frame with a tandem repeat of said individual codon, wherein said reporter polynucleotide encodes a reporter protein, and wherein said synthetic construct is operably linked to a regulatory polynucleotide and measuring expression of said reporter protein in said cell to determine the translational efficiency of said codon.

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