Method and nucleic acid for the preparation of lecithin:choleste

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435803, 4351723, 436501, 536 27, 530397, 935 78, C12Q 168

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active

050494880

ABSTRACT:
Nucleic acid encoding lecithin:cholesterol acyltransferase is ligated into expression vectors and used to transform host cells for the synthesis of lecithin:cholesterol acyltransferase in recombinant cell culture. Lecithin:cholesterol acyltransferase amino acid sequence variants are described for enhancing the properties of lecithin:cholesterol acyltransferase. Lecithin:cholesterol acyltransferase and its variants are employed in the therapy of conditions characterized by hypercholesterolemia and for the mobilization of cholesterol in vivo.

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patent: 4499184 (1985-02-01), Goodhue
Wood, W. I. et al., Nature 312, Nov. 1984, pp. 330-337.
Boguski, M. S. et al., Proc. Natl. Acad. Sci. U.S.A., 82, Feb. 1985, pp. 992-996.
McLean, J. W. et al., J. Biol. Chem. 259 1984, pp. 6498-6504.
McLean, J. et al., Nucleic Acids Res 14, 1986, pp. 9397-9406.
Chem. Abst. 90 No. 15, issued 4/9/79, p. 447 #119261j, Dobiasova, M. et al., "Lecithin:Cholesterol Acyltransferase . . . Heart Disease".

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